Test Directory

• 7q22
• 7q31.2

-7 and7q- are seen in myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML). They may occur de novo or be secondary to an exposure to chemical mutagens, chemotherapy treatments with alkylating agents, or probably secondary to immunosuppressive therapy for severe aplastic anemia. Deletion (7q) is always interstitial with a cluster of breakpoints from 7q11 to 7q36. FISH for abnormalities of chromosome 7 can be performed on interphase or metaphase cells.

Specimen Types: Leukemic Blood or Bone Marrow

Preferred Collection Container: Green Top (Sodium Heparin)

Alternate Collection Container: Lavendar Top (EDTA)

Desired Specimen Volume: 3 - 5 mL

Minimum Acceptable Volume: 1 mL

• Lithium heparin is not acceptable.
• Specimens must be collected in a sterile collection container. Do not open tube.

Each specimen must be accompanied by a requisition form, script, electronic order or physician note. Each order must indicate the reason for testing (diagnosis).

• Transport to the laboratory at room temperature.
• Store specimen as whole blood, in original tube, at room temperature and deliver to lab within 24 hours.
• If specimen cannot be delivered within 24 hours, store in refrigerator. Do not freeze specimen.
• Specimens collected on the weekends must be refrigerated upon arrival at HNL Lab Medicine.

• Specimen frozen
• Specimen arriving without any form of patient identification (unlabeled)
• Specimen with attached needles
• Specimen arriving with broken or leaking collection tube
• Unfixed specimen treated with fixative

In a normal cell, the expected signal pattern is two red signals and two green signals. In an abnormal cell containing the deletion, the expected signal patterns are one red signal and two green signals or one red signal and one green signal.

• FISH Deletion 7q
• Del(7q)