Test Directory

Addison's disease (chronic primary adrenal insufficiency) is commonly caused by autoimmune destruction of the adrenal cortex and is characterized by the presence of adrenal cortex autoantibodies in the serum. It can occur sporadically or in combination with other autoimmune endocrine diseases that together comprise Type I or Type II autoimmune polyglandular syndrome. The microsomal autoantigen, steroid 21-hydroxylase enzyme, has been shown to be the primary autoantigen associated with autoimmune Addison's disease. Measurement of 21-hydroxylase autoantibodies aids in diagnosis and management of patients with adrenal insufficiency.

Specimen Type: Serum

Collection Container: Gold Top, Serum Separator

Alternate Collection Container: Red Top, Plain

Desired Specimen Volume: 0.5 mL

1. Collect a gold top, serum separator via venipuncture.

1. Separate serum from cells within one hour of collection.
2. Transfer 0.5 mL of serum or plasma to a LabCorp PP transpak frozen purple tube.
3. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Transport to the laboratory frozen.

• Lipemic
• Grossly hemolyzed

See patient report.

• Adrenal 21-Hydroxylase Autoantibodies