Test Directory

The anti-PLA2R ELISA (IgG) test is intended for the qualitative or semiquantitative determination of IgG class autoantibodies against phospholipase A2 receptor (PLA2R) in human serum. It is used as an aid in the diagnosis of primary membranous glomerulonephritis (pMGN), in conjunction with other laboratory and clinical findings.

Specimen Type: Serum

Collection Container: Gold Top, Serum Separator

Desired Specimen Volume: 0.5 mL

1. Collect specimen in gold top, serum separator tube via venipuncture

1. Separate serum from cells.
2. Transfer 0.5 mL of serum to a clear aliquot tube.

Transport to the laboratory refrigerated. 

 See patient report.

The test is not a standalone test but an adjunct to other clinical information. A diagnosis of membranous nephropathy (MN) should not be made on a single test result. The clinical symptoms, results on physical examination, and laboratory tests (eg, serological tests), when appropriate, should always be taken into account when considering the diagnosis of primary versus secondary MN.

Absence of circulating PLA2R autoantibodies does not rule out a diagnosis of PLA2R-related membranous nephropathy. Clinical evidence must not be overruled in favor of a numerical value.

The assay has not been demonstrated to be effective for monitoring the stage of disease or its response to treatment.

Borderline samples should be considered as potentially positive and retested.

• Antibodies to the M-type Phospholipase A2 Receptor