Test Directory

• Perinuclear (P-ANCA)
• Atypical pANCA
• Anti-MPO Antibodies
• Anti-PR3 Antibodies
• Cytoplasmic (C-ANCA)

For diagnosis and monitoring inflammatory activity in primary systemic small vessel vasculitides. The anti-PR3-ANCA Immunoassay is useful for confirming positive ANCA results by IFA, particularly with the cANCA pattern. Presence of anti-PR3 antibodies is highly specific for Wegener granulomatous (WG) disease, for which the sensitivity is reported to be 98%. Levels of anti-PR3 are elevated during active phases of disease and lower during remission. Monitoring anti-PR3 levels, therefore, can aid in disease management. The anti-MPO-ANCA Immunoassay is useful for confirming positive ANCA results by IFA, particularly with the P-ANCA pattern. Presence of anti-MPO antibodies is highly specific for idiopathic and vasculitis-associated crescentic glomerulonephritis, classic polyarteritis nodosa, Churg-Strauss syndrome, and polyangiitis overlap syndrome without renal involvement. Levels of anti-MPO are elevated during active phases of disease and lower during remission. Therefore, monitoring anti-MPO levels can aid in disease management.

Specimen Type: Serum

Collection Container: Gold Top, Serum Separator or Red Top, Plain

Desired Specimen Volume: 3 mL

1. Collect a gold top, serum separator tube via venipuncture.

1. Separate serum from cells.
2. Transfer 3 mL of serum to a clear aliquot tube (screw cap).

Transport to the laboratory refrigerated. 

• Hemolysis
• Lipemia
• Gross bacterial contamination

See patient report.

• MPO-ANCA
• PR3-ANCA