Test Directory

Used to monitor Asparaginase therapy for sufficient enzyme activity and to assess silent inactivation by patient sensitization in patients who have been treated with Asparlas®, Erwinaze®, Oncaspar® or Rylaze®.

Specimen Type: Serum

Collection Container: Gold Top, Serum Separator

Alternate Collection Container: Red Top, Plain

Desired Specimen Volume: 2 mL

1. Collect a gold top, serum separator tube via venipuncture.
2. Let the sample rest for at least 30 minutes.

**Submit with completed ASPARAGINASE REQUISITION FORM**

1. Separate serum from cells within 2 hours of collection
2. Transfer 2 mL of serum to a clear aliquot tube (screw cap).
3. Freeze sample immediately.
4. Transport frozen with completed requisition

Transport to the laboratory frozen.

• Gross hemolysis
• Gross lipemia
• Icteremia

See patient report.

Measurement of samples are made against a standard curve generated from E. coli L‐asparaginase with each run. Calculations for Erwinaze®, Oncaspar®, Asparlas, and Rylaze® were empirically derived and utilize a correction factor that has been correlated to the E. coli L‐asparaginase activity.

L-Asparaginase Activity