Test Directory

Test Code
OBAR
Test Name
Barbiturates, Quantitative, Oral Fluid
CPT Codes
AMA 80345, CMS G0480
Expected Turnaround Time
1 - 5 days
Clinical Utility

Useful in determining the use or abuse of Barbiturates in oral fluid compliance monitoring.

Testing includes identification and quantitation of:

  • Secobarbital
  • Butalbital
  • Phenobarbital
Specimen and Container Info

Preferred Specimen Type: Oral Fluid

Preferred Collection Container: Intercept®i2he™ Oral Fluid Collection Device

Desired Specimen Volume: 1 mL

Collection Instructions
  1. Confirm the donor has not had anything in their mouth for 10 minutes prior to providing the oral fluid sample. If the donor has had anything in their mouth within the last 10 minutes, wait 10 minutes prior to the collection process.
  2. Instruct the donor to remove the device from the side of the pouch labeled #1 by grabbing the plastic shaft of the collection pad. Ensure the donor does not touch the pad.
  3. Instruct the donor to place the collection pad under the tongue with white side of the stick facing upward. Instruct the donor not to bite down on the device but to grip between the lips.
  4. Instruct the donor to keep the pad under the tongue until the indicator turns blue.
  5. If the collection time reaches 15 minutes and the indicator has still not turned blue, stop the collection and proceed to step 6.
  6. Instruct the donor to remove the device from their mouth and slowly place the collection device into the vial (side of pouch labeled #2).
  7. Cap the vial.
Processing and Submission Instructions
  • Interpretation is dependent on providing prescribed medication history.
  • List only medications taken in the last 2-3 days.
  • If using paper requisition, submit the specimen with a completed Oral Fluid- Compliance Drug Monitoring Requisition form (HNL-66).
Transport Instructions

Transport to the laboratory refrigerated.

Stability Requirements

Room Temperature

21 Days

Causes for Rejection

Specimens received in any container other than the Intercept®i2he™ Oral Fluid Collection Device will be rejected.

Methodology
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reference Intervals
  • Interpretation and concentration of drug/metabolite in oral fluid is dependent on dose, time of dose, and metabolic rate.
  • Reported results are corrected for the collection device dilution.
Limitations

Analyte

Cutoff Concentration (ng/mL)

Secobarbital

60

Butalbital

60

Phenobarbital

60

Performing Location
Roble Road Core Laboratory
Alternate Names
  • Butalbital
  • Fioricet
  • Luminal
  • Phenobarb
  • Phenobarbital
  • Phenobarbitone
  • Secobarbital
  • Seconal
  • Solfoton 
Testing Schedule
Routine, 2 times per week