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Test Code
FSBC6
Test Name
BCL6, 3q27.3 Gene Rearrangement by FISH (Heme)
CPT Codes
88237, 8837726, 88377TC
Included Tests

BCL6, 3q27.3
Expected Turnaround Time
3-5 business days
Clinical Utility

The B-cell lymphoma 6 (BCL6) gene is localized to 3q27. Rearrangements of 3q27 may be seen in case diffuse large B-cell lymphoma (30-40%) and follicular lymphoma (5-15%). Structural rearrangements include translocations and microdeletions, with about one-half of the translocations involving the immunoglobulin genes at 14q32, 2p12 and 22q13. The remaining translocations involve 3q27 with a wide variety of other chromosome partners. FISH can detect this rearrangement in either interphase or metaphase cells.
Specimen and Container Info

Specimen

Containers

Volume

Leukemic Blood

Preferred Collection Container: Green Top (Sodium Heparin)

Alternate Collection Container: Lavendar Top (EDTA)

Preferred Collection Volume: 3 - 5 mL

Minimum Acceptable Volume: 1 mL

Bone Marrow

Preferred Collection Container: Green Top (Sodium Heparin)

Alternate Collection Container: Lavendar Top (EDTA)

Preferred Collection Volume: 3 - 5 mL

Minimum Acceptable Volume: 1 mL

Lymph Node

Sterile Collection Container with Supporting Media
Collection Instructions
  • Lithium heparin is not acceptable.
  • Specimens must be collected in a sterile collection container.
Processing and Submission Instructions

Each specimen must be accompanied by a requisition form, script, electronic order or physician note. Each order must indicate the reason for testing (diagnosis).
Transport Instructions
  • Transport to the laboratory at room temperature.
  • Store specimen as whole blood, in original tube, at room temperature and deliver to lab within 24 hours.
  • If specimen cannot be delivered within 24 hours, store in refrigerator. Do not freeze specimen.
  • Specimens collected on the weekends must be refrigerated upon arrival at HNL Lab Medicine.
Stability Requirements

Room Temperature

24 hours

Refrigerated

72 hours

Frozen

Do not freeze
Causes for Rejection
  • Specimen frozen
  • Specimen arriving without any form of patient identification (unlabeled)
  • Specimen with attached needles
  • Specimen arriving with broken or leaking collection tube
  • Unfixed specimen treated with fixative
Methodology
FISH, Fluorescence In-Situ Hybridization
Reference Intervals

In a normal cell, the expected signal pattern is two fusion signals. In an abnormal cell containing a rearrangement, the expected signal pattern is one red signal, one green signal, and one fusion signal.
Performing Location
Roble Road Core Laboratory
Alternate Names
  • FISH BCL6 Breakapart, Heme
  • BCL6 BA
  • BCL6
  • B-Cell Lymphoma 6
Testing Schedule
Routine, Monday - Friday no holidays
Disease Group
Cytogenetic Tests
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
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