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Test Code
BENZ
Test Name
Benzodiazepines, Quantitative, Whole Blood
CPT Codes
AMA 80346, CMS G0480
Expected Turnaround Time
5 - 7 days
Clinical Utility

Useful in determining the use or abuse of Benzodiazepines.

Testing includes identification and quantitation of: 

  • Alprazolam 
  • Alpha - hydroxyalprazolam (Alprazolam Metabolite) 
  • Clonazepam
  • 7 - aminoclonazepam (Clonazepam Metabolite)
  • Diazepam
  • Lorazepam
  • Nordiazepam
  • Oxazepam
  • Temazepam
  • Midazolam
  • Etizolam
Specimen and Container Info

Specimen

Container

Volume

Whole Blood

Grey Top* (Sodium Fluoride, Potassium Oxalate)

3 mL

Whole Blood

Dark Green Top (Lithium Heparin)

3 mL

Whole Blood

Lavender Top (EDTA)

3 mL

Serum

Red Top, Plain

1 mL

*Preferred Specimen
Processing and Submission Instructions

For forensic or legal purposes, submit specimen in a plastic evidence bag with a Chain-of-Custody Form.
Transport Instructions

Transport to the laboratory refrigerated. 
Stability Requirements

Refrigerated

 7 days
Causes for Rejection

Specimens collected in separator tubes will be rejected.  
Methodology
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reference Intervals

 Analyte

Therapeutic Range (ng/mL)

Alprazolam

10 - 100

Alpha - hydroxyalprazolam

Not Established

Clonazepam

10 - 75

7 - aminoclonazepam

20 - 150*

Diazepam

100 - 1000

Lorazepam

50 - 240

Nordiazepam

100 - 1000

Oxazepam

200 - 1400

Temazepam

200 - 1100 Peak Concentration

Midazolam

50 - 600

Etizolam

Not established

*Following a 6 mg/day of Clonazepam
Limitations

Analyte

Reporting Limit (ng/mL)

Alprazolam

5

Alpha - hydroxyalprazolam

5

Clonazepam

2

7 - aminoclonazepam

10

Diazepam

20

Lorazepam

5

Nordiazepam

20

Oxazepam

20

Temazepam

20

Midazolam

5

Etizolam

2
Performing Location
Roble Road Core Laboratory
Alternate Names

Alprazolam Alternate Names:

  • Alprazolam
  • Alphahydroxyalprazolam
  • Xanax
  • Alprax
  • Alzam
  • Tranax

Clonazepam Alternate Names:

  • Clonazepam
  • 7-aminoclonazepam
  • Klonopin
  • Rivitrol

Diazepam Alternate Names:

  • Valium
  • Diastat
  • Diazepam
  • Nordiazepam 

Lorazepam Alternate Names:

  • Ativan
  • Loreev XR
  • Lorazepam

Oxazepam Alternate Names:

  • Serax
  • Oxazepam

Temazepam Alternate Names:

  • Restoril
  • Normison
  • Nortem
  • Temazepam

Midazolam Alternate Names:

  • Versed
  • Seizalam
  • Midazolam
  • Alphahydroxymidazolam

Etizolam Alternate Names:

  • Etizest
  • Etilaam
  • Etizex
  • Depas
  • Sedekopan
  • Pasaden
Testing Schedule
Routine, 2 times per week
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
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