Test Directory

Drug concentrations are used to assess and adjust medication dosage to optimize therapeutic effectiveness while minimizing adverse effects.

Specimen Type: Plasma

Collection Container: 6 Timed Collection, Green Top (Sodium Heparin)

Desired Specimen Volume: 4 mL per tube (24 mL total)

**FOR STEM CELL TRANSPLANT ORDERS ONLY**

1. Testing must be scheduled 48 hours in advance and Referral Testing must be notified.
2. Collect 6 green top (sodium heparin) tubes via venipuncture.
3. Samples must remain refrigerated at all times or kept in wet ice slurry.

1. Separate plasma from cells ASAP in a refrigerated centrifuge.
2. For adult patients, transfer 4 mL of plasma from each collection container into 6 separate clear aliquot tubes (screw caps) and freeze immediately.
3. For pediatric patients, transfer 1 mL of plasma from each collection container into 6 separate clear aliquot tubes (screw caps) and freeze immediately.
4. Transport all specimens in a small Styrofoam container with dry ice.
5. Specimens must arrive in referral testing by 1600.
6. Submit sample with a IV Q6 BUSULFAN PHARMACOKINETICS REQUISITION that has been signed by the attending physician or designee/caregiver.

Referral Testing:

• Advance notice of shipment (at least 48 hours) is required by Fred Hutch Cancer Center.
• Complete the Busulfan Testing Web Order Form and email pklab@fredhutch.org the completed web form, requisition, and tracking number for the sample being sent.
• Samples must be shipped on a minimum of 3 kg of dry ice using an overnight carrier (XL white shipping box).

Transport to the laboratory frozen. 

• Refer to Busulfan Sample Rejection Criteria for Referral Clients

See patient report.

Busulfan degrades quickly at room temperature.