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Test Code
CALRN
Test Name
Calreticulin Exon 9 Mutation Analysis
CPT Codes
81219
Expected Turnaround Time
7 days
Clinical Utility

Calreticulin (CALR) mutation analysis aids diagnostic confirmation of Philadelphia-chromosome negative and JAK2/MPL-mutation negative myeloproliferative neoplasm (MPN). CALR mutations are mutually exclusive with JAK2 and MPL mutations. CALR mutations are detected in peripheral blood in the majority (~70-85%) of essential thrombocythemia (ET) and primary myelofibrosis (PMF) cases that are JAK2- and MPL- mutation negative. CALR mutations are not reported in polycythemia vera (PV) and can distinguish ET and PMF from PV. Presence of CALR mutations is also associated with a better clinical course than JAK2 mutations.
Specimen and Container Info

Specimen

Container

Preferred Volume

Minimum Volume

Peripheral Blood

Lavender Top (EDTA)

5 mL

3 mL

Bone Marrow

Lavender Top (EDTA)

2 mL

1 mL
Processing and Submission Instructions
Ship same day as drawn whenever possible.
Transport Instructions

Transport specimen to laboratory refrigerated.
Stability Requirements

Refrigerated

72 hours
Causes for Rejection
All specimens will be rejected if they are frozen.
Methodology
Next Gen Sequencing (NGS)
Performing Location
Roble Road Core Laboratory
Alternate Names
  • CALR
  • CALR Mutation Analysis
  • Calreticulin
  • CALR Mutation Analysis by NGS
Reference Lab
Neogenomics Laboratories, Inc.
Testing Schedule
Routine, Monday - Friday no holidays
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
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