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Test Code
CHPTB
Test Name
Chimerism, Posttransplant, B Cells
CPT Codes
81268
Expected Turnaround Time
5 - 10 days
Clinical Utility
Use to monitor engraftment of donor cells post allogeneic stem cell transplant.
Specimen and Container Info

Specimen

Container

Volume

Whole Blood

Lavender Top EDTA Tube; OR Pale Yellow Top (ACD) Tube

2 mL

Bone Marrow

Lavender Top (EDTA) Tube

1 mL
Collection Instructions

Whole Blood:

  1. Collect whole blood in lavender top (EDTA) tube or pale yellow top (ACD) tube via venipuncture
  2. Transport specimen in original collection container immediately

Bone Marrow:

  1. Collect bone marrow in lavender top (EDTA) tube via bone marrow collection procedure
  2. Transport specimen in original collection container immediately
Processing and Submission Instructions

**FOR STEM CELL TRANSPLANT ORDERS ONLY**

**REQUIRES "Cell Isolation Request for Chimerism, Posttransplant, Sorted Cells" FORM**
Transport Instructions

Transport to the laboratory refrigerated. 
Stability Requirements

Room Temperature

72 hours

Refrigerated

72 hours
Causes for Rejection
Clotted or hemolyzed specimens.
Methodology
Polymerase Chain Reaction (PCR)/Fragment Analysis/Immunomagnetic Cell Separation
Reference Intervals

Background Information: Chimerism, Posttransplant, Sorted Cells (B Cells)

Indication: Monitoring for bone marrow transplant patients; correlation with clinical status and consideration of the interval between bone marrow transplantation and testing is necessary for proper interpretation of results.

Methodology: PCR followed by capillary electrophoresis. Specimens are analyzed using 15 autosomal markers (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, THO1, D13S317, D16S539, D2S1338, D19S433, vWa, TPOX, D18S51, D5S818, and FGA) and one gender marker (amelogenin).

Limit of Detection: 2 percent of minor cell population.

Limitations: Diagnostic errors can occur due to rare sequence variations.
Reference Lab
ARUP Laboratories
Testing Schedule
Routine, daily
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
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