Test Directory

• ABO
• Rh
• Direct Coombs Test IgG

Reflexed Tests

• Eluate
• Antibody Screen
• Antibody Identification
• Phenotype Antigen Test

Cord blood testing will be performed on all babies born to Rh (D) negative women, and any woman known to be sensitized against a clinically significant red cell antigen. Rh (D) negative women who deliver Rh (D) positive babies should receive Rh (D) Immune Globulin (RhIG), provided allo anti-D is not present.

An eluate may be performed in cases of clinically significant maternal antibodies.

Lehigh Valley Health Network

Preferred Specimen Type: Cord Blood

Preferred Collection Container: 1 Pink Top (EDTA)

Alternate Collection Container: 2 Lavender Top (EDTA)

Desired Specimen Volume: 6 mL

Minimum Acceptable Volume: 3 mL

Redeemer Health

Preferred Specimen Type: Cord Blood

Preferred Collection Container: 1 Red Top

Desired Specimen Volume: 6 mL

Minimum Acceptable Volume: 3 mL

Lehigh Valley Health Network

The specimen tube shall be properly labeled with the following 3 independent identifiers for inpatients and outpatients:

1. Patient's full name
2. Date of birth
3. Medical record number

The labels shall also include the following verifiers:

1. Date and time of collection
2. Initials/tech code of phlebotomist

Redeemer Health

The specimen tube shall be properly labeled with a chart label from baby and mom. The following 2 independent identifiers are required for both baby and mom:

1. Patient's full name
2. Medical record number

The label shall also include the following verifiers:

1. Date and time of collection
2. Full name/tech code of nurse/doctor

Unique patient identifiers (name and medical record number) must not be changed or added once the tube has been labeled at the patient's bedside

i. Transport to the laboratory at room temperature within 24 hours of collection

ii. Transport to the laboratory refrigerated past 24 hours of collection

• Insufficient volume
• Inappropriate specimen type

• Negative DAT: Red cells do not have any bound antibody and/or complement
• Positive DAT: Suggests the presence of in vivo bound complement and/or antibody

Positive DAT IgG