Test Directory

• Desmoglein 3 IgG
• Desmoglein 1 IgG

This test is a preferred screening test for patients suspected to have an autoimmune blistering disorder of the skin or mucous membranes (pemphigus). This test aids in the diagnosis of pemphigus. The antibody levels can be used to monitor the effectiveness of drug treatment.

Pemphigus diseases involve an autoimmune response primarily targeting desmoglein (Dsg) 1 and 3, which are cadherin-type transmembrane adhesion molecules. These desmogleins, in conjunction with desmocollins, play a crucial role in maintaining the cohesion between epidermal keratinocytes and are connected internally to the intermediate filament network through various plakins.

Pemphigus foliaceus (PF) is characterized by IgG autoantibodies against Dsg1, resulting in blistering limited to the upper skin, with no evident involvement of mucous membranes. On the other hand, pemphigus vulgaris (PV) primarily targets Dsg3 as the major autoantigen, but approximately 50% to 60% of patients also develop additional autoantibodies against Dsg1, leading to significant mucous membrane involvement.

Specimen Type: Serum

Collection Container: Gold Top, Serum Separator

Alternate Collection Container: Red Top, Plain

Desired Specimen Volume: 2 mL

1. Collect a gold top, serum separator tube via venipuncture.

1. Separate serum from cells.
2. Transfer 2 mL of serum to a LabCorp PP transpak frozen purple tube.

Transport to the laboratory frozen. 

• Bacterial contamination
• Hemolysis
• Lipemia

 See patient report.

• Desmoglein 1 and Desmoglein 3 (Pemphigus), IgG Antibodies
• Desmoglein 1 and 3 IgG
• Pemphigus Autoantibodies