Test Directory

• Polyspecific Anti-IgG and Anti-C3d
• Monospecific Anti-IgG
• Monospecific Anti-C3d

Reflexed when appropriate:

• ABO
• Rh

Positive results may be found in autoimmune hemolytic anemia, acute and delayed hemolytic transfusion reactions, hemolytic disease of the newborn, and may be drug-induced or idiopathic in nature.

Preferred Collection Container: 1 Pink Top (EDTA)

Alternate Collection Container: 2 Lavender Top (EDTA)

Desired Specimen Volume: 6 mL

Minimum Acceptable Volume: 3 mL

Other Instructions: Minimum volume for infants <4 months is 2 full Microtainer, Lavender Top (EDTA)

Lehigh Valley Health Network

The specimen tube shall be properly labeled with the following 3 independent identifiers for inpatients and outpatients:

1. Patient's full name
2. Date of birth
3. Medical record number

The labels shall also include the following verifiers:

1. Date and time of collection
2. Initials/tech code of phlebotomist

Exception Criteria:

• Trauma -ED patient: Patients with unknown name and date of birth, medical record number and temporary name are acceptable.
• Good Shepherd Rehab: Typenex band ID is used as an additional patient identifier

Redeemer Health

The specimen shall be identified at the time of collection and labeled at bedside with:

1. Full name of patient exactly as it appears on the patient's identification bracelet
2. Medical record number
3. Phlebotomy code of the person who drew the specimen. If they do not have a code, full name printed legibly.
4. Date and time of collection

Unique patient identifiers (name and medical record number) must not be changed or added once the tube has been labeled at the patient's bedside.

Non-hospitalized patients: Submit specimen with a completed Blood Bank Requisition (LAB-04) Form. Transfusion and pregnancy history within the last 3 months should be included.

i. Transport to the laboratory at room temperature within 24 hours of collection

ii. Transport to the laboratory refrigerated past 24 hours of collection

• Mislabeled
• Insufficient volume
• Inappropriate specimen type

• Negative DAT: Red cells do not have any bound antibody and/or complement
• Positive DAT: Suggests the presence of in vivo bound complement and/or antibody

• Neonatal positive Anti-IgG