Test Directory

Test Code
ENDOG
Test Name
Endomysial Antibody, IgG
CPT Codes
86231
Expected Turnaround Time
2 - 3 days
Clinical Utility

Serological methods of detecting Immunoglobulin A (IgA) antibodies to gliadin, endomysium (EMA), reticulin, and tissue transglutaminase are routinely used for diagnosing both symptomatic and asymptomatic patients with Celiac Disease (CD). Since Immunoglobulin A (IgA) deficiency is 10 to 15 times more common in patients with Celiac Disease than in healthy subjects, IgG-specific antibody tests for endomysium are useful for the identification of IgA-deficient patients with CD.

Specimen and Container Info

Specimen Type: Serum

  

Collection Container: Gold Top, Serum Separator

Desired Specimen Volume: 2 mL

Collection Instructions
  1. Collect a gold top, serum separator tube via venipuncture.
Processing and Submission Instructions
  1. Allow the blood to clot in an upright position for at least 30 minutes but not longer than 1 hour before centrifugation. 
  2. Separate serum from cells within 1 hour of collection.
  3. Transfer 2 mL of serum to a clear aliquot tube (screw cap).
Transport Instructions

Transport to the laboratory frozen.

Stability Requirements

Room Temperature

48 hours

Refrigerated

14 days

Frozen

30 days

Causes for Rejection
  • Gross hemolysis
  • Lipemia
  • Microbially contaminated specimens
  • Specimens received outside of stability
Methodology
Immunofluorescence Assay
Reference Intervals

See patient report.

Limitations

This test is used for clinical purposes.

Reference Lab
Quest Diagnostics Nichols Institute
Testing Schedule
Monday-Friday