Test Directory

• Fungal PCR: Summary
• Detection, 28S rDNA
• 28S Identification
• Detection, ITS rDNA
• ITS Identification
• Specimen DNA Extraction

Gene sequencing of 28S rRNA gene for molds and ITS for both molds and yeasts provides rapid, accurate identification of clinically significant fungi. The regions contain conserved regions useful for the design of universal primers that amplify the gene from all pathogenic and nonpathogenic fungi in addition to hypervariable regions that contain species-specific signature sequences useful for fungal identification to species level.

1. Collect tissue or fluid in a sterile container.

**MICRO AND INFECTIOUS DISEASE USE ONLY**

Requires University of Washington Molecular Microbiology Test Requisition Form

1. Transport specimen in the original collection container.
2. For FFPE specimens, a pathology report is recommended to be sent with the sample.

Transport to the laboratory frozen. 

• Sputum
• Blood, serum, plasma, stool/rectal swabs
• No citrated or heparinized solutions
• Tissues floating in formalin
• Swab/fluid collected in tube containing agar

See patient report.

This test may have interference due to some lots of eSwabs which have been found to contain Saccharomyces cerevisiae DNA, resulting in false positive detection. Clinical correlation and/or retesting with a different collection method is advised. The detection of S. cerevisiae from eSwab specimens can interfere with our ability to rule out other fungal DNA.

Formalin fixation dramatically reduces the sensitivity of the assays due to reduced template yield and quality. Fresh tissue is the optimal specimen of choice, as it reduces the chance of introducing exogenous DNA templates or microorganisms during embedding/fixation.

• 28s PCR
• 28S sequencing
• Broad range fungal PCR
• Broad range fungi PCR
• Fungal 28S
• Fungal identification
• Fungal ITS
• Fungal PCR
• Fungal sequencing
• Fungi identification
• Fungi PCR
• Fungi sequencing
• ITS PCR
• ITS sequencing
• Molecular fungal
• Molecular fungi
• Universal fungal PCR
• Universal fungi PCR