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Test Code
HBVLD
Test Name
Hepatitis B Virus by Quantitative PCR
CPT Codes
87517
Included Tests
  • HBV Viral Load, IU/mL
  • HBV Viral Load, log IU/mL
Expected Turnaround Time
1 - 7 days
Clinical Utility

This test is used for the quantitation of hepatitis B virus (HBV) in human serum or plasma. It is used as an aid in the management of patients with chronic HBV infection undergoing antiviral therapy. The test can be used to measure HBV DNA levels at baseline and during treatment to aid in assessing response to treatment.
Specimen and Container Info

Specimen Type: Plasma; Serum

Collection Container:  Lavender Top (EDTA); Gold Top, Serum Separator

Desired Specimen Volume:  2 mL

Minimum Acceptable Volume:  1 mL
Collection Instructions
  1. Within 24 hours of collection, centrifuge for 10 min <1300 rcf.
  2. For Lavender Top (EDTA) tubes, aliquot plasma into a sterile plastic tube.
  3. For Gold Top, Serum Separator tubes, do not aliquot.
Transport Instructions

Transport to the laboratory refrigerated. 
Stability Requirements

Room Temperature

Whole Blood, Plasma and Serum, 24 Hours

Refrigerated

Whole Blood, 24 Hours; Plasma and Serum, 6 Days

Frozen

Plasma and Serum, 6 Months at -70C

Plasma and Serum, 12 Weeks at -20C

Freeze/Thaw Cycles

 4 Freeze / Thaw
Causes for Rejection
  • Any specimen with leakage on the outside of the container will be rejected. Ensure the lid is tightly closed.
  • Any specimen shared with another laboratory test will be rejected.
  • Any specimen collected in an outdated collection tube will be rejected.
Methodology
Quantitative Polymerase Chain Reaction (qPCR)
Reference Intervals

HBV Virus Not Detected
Limitations

Technical Range of Assay

10 - 1.0 x 1,000,000,000 IU/mL

1.00 - 9.00 log IU/mL
Performing Location
Roble Road Core Laboratory
Alternate Names
  • HBV DNA Quantitative by PCR
  • Hepatitis B Viral Load
  • HBV Viral Load
Testing Schedule
Routine, 1 time per week
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
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