Test Directory

• HSV-1 DNA
• HSV-2 DNA

This test is intended for use as an aid in the diagnosis of herpes simplex virus (HSV) infections; it also differentiates HSV-1 from HSV-2. PCR testing of blood, serum, or plasma samples is clinically useful only in potential cases of disseminated HSV infection (neonates, immunosuppressed individuals) and not as an aid in the diagnosis of either mucosal or CNS disease.

Serum:

1. Collect a gold top, serum separator tube via venipuncture

Plasma:

1. Collect a lavender top (EDTA) tube via venipuncture

Whole Blood

1. Collect a lavender top (EDTA) tube or pale-yellow top (ACD) via venipuncture.

Swab

1. Swab the lesion utilizing an UTM.

Serum:

1. Separate serum from cells.
2. Transfer 1 mL of serum to a clear aliquot tube (screw cap)

Plasma:

1. Separate plasma from cells.
2. Transfer 1 mL of plasma to a clear aliquot tube (screw cap).

Whole Blood

1. Transport 1 mL of whole blood in the original collection container. Do not freeze.

Swab

1. Place swab into media and screw lid on securely.

Transport to the laboratory refrigerated. 

See patient report.

HSV PCR is a single test that both detects the presence of HSV DNA and determines which type is present in the positive samples. There is no mechanism, therefore, for testing for HSV-1 without simultaneously testing for HSV-2.

•  HSV Types 1 and 2, DNA PCR (Real Time)