Test Directory

IGH, 14q.32.3

Rearrangements involving the immunoglobulin heavy chain (IGH) gene have been identified in about 50% of non-Hodgkin B-cell lymphomas (NHL) and correlated to clinically relevant subgroups. A t(14;18)(q32;q21) is found in 88.1% of follicular lymphomas and a t(11;14)(q13;q32) is in 85.7% mantle cell lymphomas. IGH rearrangements are identified in 52.5% of diffuse large B-cell lymphomas. Rearrangements of the IGH gene occur in 34.7% of plasma cell myeloma cases and is among the most frequent chromosomal change. FISH can detect this rearrangement in either interphase or metaphase cells.

Specimen: Formalin-Fixed Paraffin Embedded (FFPE) Tissue Block or 2 slides from FFPET block, cut at 4 micron thickness and mounted on charged lysine slides with accompanying H&E slide indicating the region(s) of interest (ROI), if the entire specimen is not tumor.

Specimens should have an ischemia time of < 1 hour and a formalin fixation time of

6 - 72 hours.

Transport to the laboratory at room temperature.

• Specimen arriving without any form of patient identification (unlabeled)
• FFPET sections that are not properly attached to the slide
• FFPET sections with excessive cracks, splits or deterioration that would inhibit analysis
• Broken FFPET slides

In a normal cell, the expected signal pattern is two fusion signals. In an abnormal cell containing a rearrangement, the expected signal pattern is one red signal, one green signal, and one fusion signal.

• FISH IGH Breakapart
• IGH BA
• IGH
• Immunoglobulin Heavy Chain