Test Directory

Test Code
IGE
Test Name
Immunoglobulin E, Total
CPT Codes
82785
Expected Turnaround Time
3 - 5 days
Clinical Utility
Immunoglobulin E (IgE) is firmly bound to mast cells and only trace amounts are normally present in serum. When antigen (allergen) cross-links two IgE molecules, the mast cell is stimulated to release histamine and other vasoactive amines. These vasoactive amines are responsible for the vascular permeability and smooth muscle contraction occurring in such allergic reactions as hay fever, asthma, urticaria, and eczema.
Specimen and Container Info

Specimen Type: Serum

Preferred Collection Container: Gold Top, Serum Separator

Minimum Acceptable Volume: 0.2 mL

Volume reflects estimated whole blood.

Note: This volume does not allow for repeat testing.

Processing and Submission Instructions

Separator tubes: Contains clot activator and gel for separating serum from cells, but not anticoagulant. Separate serum from cells as soon as the clot has formed (30 minutes). If specimen is centrifuged before clotting is complete, a fibrin clot will form on top of the cell. This may cause the gel barrier to not be intact and could cause improper separation of serum and cells, possibly affecting test results.

Transport Instructions

Transport to the laboratory refrigerated.

Stability Requirements

Refrigerated

7 days

Frozen

Samples are frozen after testing is complete and held on-site for 2 months.

Methodology
Fluorescent Enzyme Immunoassay
Reference Intervals
Performing Location
Roble Road Core Laboratory
Testing Schedule
Routine, 6 times per week