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Test Code
IGVH
Test Name
Immunoglobulin Heavy Chain Gene Variable Region (IGVH) Mutation
Included Tests

RT-PCR and bi-directional sequencing of the variable region of the immunoglobulin heavy chain for detection of mutation from germline sequence. The mutated VH gene family is identified in positive reports (>2% sequence deviation).
Expected Turnaround Time
9 - 12 days
Clinical Utility

IgVH mutation is a significant prognostic marker in chronic lymphocytic leukemia (CLL). IgVH mutation analysis combined with FISH, TP53 mutation analysis and Beta-2 microglobulin measurement provide comprehensive prognostic assessment and may be used to determine the approach to therapy for all CLL patients.
Specimen and Container Info

Specimen

Preferred Container

Acceptable Container

Preferred Volume

Minimum Volume

Peripheral Blood

Lavender Top (EDTA)

Green Top (Sodium Heparin)

5 mL

3 mL

Bone Marrow

Lavender Top (EDTA)

Green Top (Sodium Heparin)

2 mL

1 mL
Transport Instructions

 Transport to the laboratory refrigerated.
Stability Requirements

Refrigerated

Up to 14 days
Causes for Rejection
  • Frozen specimens
  • Fixed specimens
  • Incorrect collection container
Methodology
Molecular
Limitations

Mutation may not be detectable in specimens containing <10% clonal B-cells.
Performing Location
Roble Road Core Laboratory
Alternate Names
IgVH Mutation Analysis
Reference Lab
Neogenomics Laboratories, Inc.
Testing Schedule
Routine, Monday - Friday no holidays
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
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