Test Directory

Test Code
INFXR
Test Name
Infliximab Quantitation with Antibody Reflex
CPT Codes
80230
Expected Turnaround Time
3 - 7 days
Clinical Utility

Useful for evaluating patients for loss of response, partial response on initiation of therapy, autoimmune or hypersensitivity reactions, primary nonresponse, reintroduction after drug holiday, endoscopic/computed tomography enterography recurrence (in inflammatory bowel disease), acute infusion reactions and proactive monitoring

Specimen and Container Info

Specimen Type: Serum

Preferred Collection Container: Red Top, Plain

Alternate Collection Container: Gold Top, Serum Separator

Desired Specimen Volume: 1 mL

Collection Instructions
  1. Collect a red top, plain tube via venipuncture.
Processing and Submission Instructions
  1. Separate serum from cells within 2 hours of collection.
  2. Transfer 1 mL of serum to a clear aliquot tube (screw cap).
Patient Preparation
  • Draw blood immediately before next scheduled dose (trough specimen).
  • For 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.
Transport Instructions

Transport to the laboratory refrigerated. 

Stability Requirements

Refrigerated

28 days

Frozen

28 days
Causes for Rejection
  • Gross hemolysis
  • Gross icterus
Methodology
Selective Reaction Monitoring Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reference Intervals

See patient report.

Limitations
  • Samples containing more than 12.5 ng/mL biotin (vitamin B7) may interfere (in the form of depressed signal) with INXAB / Infliximab Antibodies, Serum.
  • This test does not differentiate between the originator and biosimilar products.
Alternate Names
  • Infliximab Quantitation with Reflex to Antibodies to Infliximab, Serum
  • Remicade
Reference Lab
Mayo Clinic Laboratories
Testing Schedule
Routine, 5 times per week
Reflex Tests
INXAB
What triggers the reflex testing
When infliximab results are below 5.1 mcg/mL, testing for antibodies to infliximab will be performed at an additional charge.