Test Directory

• ABO
• Rh
• Antibody Screen
• Titer

Reflexed when appropriate:

• Antibody Identification
• Phenotype Antigen Test
• Direct Coombs Test
• Eluate
• EGA Treatment
• Human Erythrocyte Antigen (Genotype)
• Adsorption
• DTT

The following blood types indicate expected isoagglutinin (anti-A and/or anti-B):

1. AB: not present
2. A: anti-B
3. B: anti-A
4. O: anti-A, B, anti-A: anti-B

ABO incompatible kidney transplant recipients are followed by titration to determine the amount of circulating anti-A and anti-B prior to surgery and post-transplantation.

Normal titers form between the age of 3-6 months. Prior to this time the titers are passively acquired from the mother.

Preferred Specimen Type: Whole Blood

Preferred Collection Container: 1 Pink Top (EDTA)

Alternate Collection Container: 2 Lavender Top (EDTA)

Desired Specimen Volume: 6 mL

Minimum Acceptable Volume: 3 mL

Other Instructions: Minimum volume for infants <4 months is 2 full Microtainer, Lavender Top (EDTA)

The specimen tube shall be properly labeled with the following 3 independent identifiers for inpatients and outpatients:

1. Patient's full name
2. Date of birth
3. Medical record number

The labels shall also include the following verifiers:

1. Date and time of collection
2. Initials/tech code of phlebotomist

Exception Criteria:

• Trauma -ED patient: Patients with unknown name and date of birth, medical record number and temporary name are acceptable.
• Good Shepherd Rehab: Typenex band ID is used as an additional patient identifier

i. Transport to the laboratory at room temperature within 24 hours of collection

ii. Transport to the laboratory refrigerated past 24 hours of collection

• Mislabeled
• Insufficient volume
• Inappropriate specimen type

• Normal adult titer > 16