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Test Code
JAK12
Test Name
JAK 2 Exon 12-15 Mutation Analysis
CPT Codes
81279
Expected Turnaround Time
7 days
Clinical Utility
  • Next-generation sequencing to detect mutations on exons12-15 of the JAK2 gene, excluding the V617F mutation. V617F analysis is recommended before or concurrently with this test.
  • While the majority of polycythemia vera (PV) patients carry the V617F mutation (~90%), most of those who are negative carry one of over 40 additional JAK2 mutations in exons 12-15. Mutation analysis helps differentiate reactive conditions from malignant erythrocytosis.
Specimen and Container Info

Specimen

Container

Preferred Volume

Minimum Volume

Peripheral Blood

Lavender Top (EDTA)

5 mL

3 mL

Bone Marrow

Lavender Top (EDTA)

2 mL

1 mL
Transport Instructions

Transport to the laboratory refrigerated.
Stability Requirements

Refrigerated

7 days
Causes for Rejection
  • Frozen specimens
  • Incorrect specimen type
  • Incorrect specimen container
  • Specimens >7 days old before testing is initiated
Methodology
Next Gen Sequencing (NGS)
Performing Location
Roble Road Core Laboratory
Alternate Names
  • JAK2 Exon 12
  • JAK2 Exon 12-13
  • JAK2 Exon 12-15
  • JAK2 Extended (Exon12-15 non-V617F) Mutation analysis by NGS
Reference Lab
Neogenomics Laboratories, Inc.
Testing Schedule
Routine, Monday - Friday no holidays
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
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