Test Directory

Test Code
JAK2
Test Name
JAK2 (V617F) Mutation by PCR, Quantitative
CPT Codes
81270, G0452
Expected Turnaround Time
1 - 4 days
Clinical Utility

Aids in the diagnosis of myeloproliferative disorders (MPD) such as polycythemia vera (PV), essential thrombocythemia (ET), or agnogenic myeloid metaplasia (primary myelofibrosis, PMF).

Specimen and Container Info

Specimen

Containers

Volume

Whole Blood

Lavender Top (EDTA)

1 mL

Bone Marrow

Sterile Container

1 mL

Patient Preparation

 

Transport Instructions

Transport to the laboratory at room temperature. 

Stability Requirements

Room Temperature

Bone Marrow, EDTA Blood: 7 days

Refrigerated

Bone Marrow, EDTA Blood: 7 days

Freeze/Thaw Cycles

5 Freeze / Thaw

Causes for Rejection
  • Any specimen with leakage on the outside of the container will be rejected. Ensure the lid is tightly closed.
  • Any specimen shared with another laboratory test will be rejected.
  • Any specimen collected in an outdated collection vial will be rejected.
Methodology
Polymerase Chain Reaction (PCR)
Reference Intervals

JAK2 V617F Mutation Not Detected

Performing Location
Roble Road Core Laboratory
Alternate Names
  • JAK2 V617F
  • MPN JAK2 V617F
  • Mutant JAK2 V617F Allelic Burden
Testing Schedule
Routine, 3 times per week
Genes
JAK2 (V617F)