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Test Code
CKIT
Test Name
KIT (c-KIT) Mutation Analysis
Included Tests

Bi-directional sequencing of KIT exons 8, 9, 11, 13 and 17 for detection of activating mutations including the common mutation D816V.
Expected Turnaround Time
7 - 10 business days
Clinical Utility

The four tested exons encompass the majority of mutations found in gastrointestinal stromal tumors (GIST), melanoma, core-binding factor acute myeloid Leukemia (CBF-AML), mast cell disease (systemic mastocytosis), and germ cell tumors. Mutation identification is useful for planning TKI therapy and predicting clinical course.
Specimen and Container Info

Specimen

Container

Preferred Volume

Minimum Volume

Peripheral Blood

Lavender Top (EDTA)

5 mL

3 mL

Bone Marrow

Lavender Top (EDTA)

2 mL

1 mL

Fixed cytogenetic cell pellet

Sterile Container

Two pieces, 0.2 cm³ each

One piece, 0.2 cm³

FFPE tissue

Paraffin Block

Paraffin Block

1 H&E slide plus 5-10 unstained slides cut at 5 or more microns
Transport Instructions

Transport to laboratory refrigerated.
Stability Requirements

Refrigerated

72 hours
Causes for Rejection
  • Frozen specimens
  • Fresh tissue samples
  • Incorrect specimen container
Methodology
Molecular
Performing Location
Roble Road Core Laboratory
Alternate Names
  • KIT Mutation Analysis
  • cKIT
  • cKIT Mutation Analysis of Exons 8, 9, 11, 13, and 17
  • cKit D816V Mutation Analysis
Reference Lab
Neogenomics Laboratories, Inc.
Testing Schedule
Routine, Monday - Friday no holidays
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
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