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Test Code
LYTPR
Test Name
Leukemia/Lymphoma Profile, Tissue/Fluid
Included Tests
  • Markers Evaluated: CD45, CD10, CD19, CD23, CD20, CD5, KAPPA, LAMBDA, CD57, CD56, CD4, CD3, CD2, CD7, CD8, CD38, CD11b, CD11c, and CD34
  • Additional markers may be performed based on hematopathologist review.
Expected Turnaround Time
2 - 4 days
Clinical Utility

Immunophenotyping by flow cytometry distinguishes among various hematopoietic cell populations and determines the degree of expression of a panel of cell surface antigens. The test is used along with morphologic, cytogenetic, and molecular genetic analysis in the identification/classification of hematopoietic neoplasms (leukemia, lymphoma, myelodysplastic disorders, etc.).
Specimen and Container Info

Specimen

Container

Alternate Container

Volume

Tissue

RPMI

Sterile container with saline

2 0.2 cm³ Pieces

Fluid

Lavender Top (EDTA)

Sterile Container

5 mL
Collection Instructions

Room Temperature specimens are acceptable but not preferred.
Processing and Submission Instructions
  • Because non-viable cells lead to nonspecific monoclonal antibody binding, specimens should be processed as early as possible.
  • Because of the critical nature of Tissue/Fluid specimens, the laboratory will attempt to process all specimens received, regardless of specimen age with hematopathologist approval.
  • Specimens collected on a Friday should be received in the Flow Cytometry Department by 1600 to ensure same day processing.
Transport Instructions

Transport to the laboratory refrigerated.
Stability Requirements

Refrigerated

72 hours
Causes for Rejection
  • Frozen specimens
  • Fixed specimens
Methodology
Flow Cytometry
Performing Location
Roble Road Core Laboratory
Alternate Names
  • Tissue/Fluid Flow Cytometry Profile for Leukemia/Lymphoma
  • Leukemia/Lymphoma Immunophenotyping, Tissue/Fluid
Testing Schedule
Routine, Monday - Friday no holidays
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
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