Test Directory

• BCL6, 3q27.3-q28 Rearrangement
• IGH::CCND1 (BCL1), t(11;14)
• IGH ,14q32.3 Rearrangement
• IGH::BCL2, t(14;18)
• MALT1 ,18q21.31-q21.32 Rearrangement
• Reflexed when appropriate: IGK and IGL

This targeted panel is appropriate when clinical and morphologic evaluation is most suggestive of a low-grade/small B-cell lymphoma, and high-grade lymphomas (such as large cell anaplastic and Burkitt lymphoma) are not a diagnostic consideration. It detects genomic changes associated with low-grade lymphomas: Follicular lymphoma, Mantle cell lymphoma (MCL), and Marginal zone lymphoma (MZL)/MALT lymphomas. BCL6 evaluation (included in this panel) can provide further prognostic information in cases of follicular lymphoma since it can indicate a higher risk for transformation to aggressive lymphoma.

Specimen: Formalin-Fixed Paraffin Embedded (FFPE) Block or 6 slides from FFPET block, cut at 4 micron thickness and mounted on charged lysine slides with accompanying H&E slide indicating the region(s) of interest (ROI), if the entire specimen is not tumor.

Specimens should have an ischemia time of < 1 hour and a formalin fixation time between 6 - 72 hours.

Transport to the laboratory at room temperature.

• Specimen arriving without any form of patient identification (unlabeled)
• FFPET sections that are not properly attached to the slide
• FFPET sections with excessive cracks, splits or deterioration that would inhibit analysis
• Broken FFPET slides

BCL6, 3q27 Breakapart Probe-Rearrangement

In a normal cell, the expected signal pattern is two fused signals. In an abnormal cell with a rearrangement, the expected signal pattern is one red signal, one green signal, and one fused signal.

BCL1(CCND1)::IGH, t(11;14) Translocation Probe

In a normal cell, the expected signal pattern is two red signals and two green signals. In an abnormal cell containing a typical translocation, the expected signal pattern is one red signal, one green signal, and two fused signals.

IGH, 14q32.3 Breakapart Probe- Rearrangement

In a normal cell, the expected signal pattern is two fused signals. In an abnormal cell with a rearrangement, the expected signal pattern is one red signal, one green signal, and one fused signal.

IGH::BCL2, t(14;18) Translocation Probe

In a normal cell, the expected signal pattern is two red signals and two green signals. In an abnormal cell containing a typical translocation, the expected signal pattern is one red signal, one green signal, and two fused signals.

MALT1, 18q21.3 Breakapart Probe-Rearrangement

In a normal cell, the expected signal pattern is two fused signals. In an abnormal cell with a rearrangement, the expected signal pattern is one red signal, one green signal, and one fused signal.

• FISH NHL B-Cell Low Grade Lymphoma Panel, Tissue

• Associations:
• Diffuse Large B-cell Lymphoma (DLBCL)
• Follicular Lymphoma (FL)
• Mantle Cell Lymphoma (MCL)