Test Directory

Test Code
NLYWB
Test Name
Lyme Disease Ab, Western Blot
CPT Codes
86617 X 2
Included Tests
  • B. burgdorferi IgG Antibody
  • B. burgdorferi IgM Antibody
  • p30 BB IgG
  • p93 BB IgG
  • p18 BB IgG
  • p23 BB IgG
  • p28 BB IgG
  • p39 BB IgG
  • p41 BB IgG
  • p45 BB IgG
  • p58 BB IgG
  • p66 BB IgG
  • p23 BB IgM
  • p39 BB IgM
  • p41 BB IgM
  • Interpretation
Expected Turnaround Time
3 - 7 days
Clinical Utility

Both ELISA IgM and IgG antibody responses to B. burgdorferi in human serum, are confirmed by using an immunoblot (IB) method where whole-cell sonicate (WCS) B. burgdorferi antigens are utilized in identifying B. burgdorferi specific antigen binding reactivity.

Specimen and Container Info

Preferred Specimen Type: Serum

Desired Specimen Volume: 2 mL

Preferred Collection Container: Gold Top, Serum Separator

Processing and Submission Instructions

Separator tubes: Contains clot activator and gel for separating serum from cells, but not anticoagulant. Separate serum from cells as soon as the clot has formed (30 minutes). If specimen is centrifuged before clotting is complete, a fibrin clot will form on top of the cell. This may cause the gel barrier to not be intact and could cause improper separation of serum and cells, possibly affecting test results.

Transport Instructions

Transport refrigerated.

Stability Requirements

Refrigerated

7 days

Frozen

<20°C for long-term storage.

Freeze/Thaw Cycles

Avoid multiple freeze/thaw cycles.

Methodology
Immunoblot
Reference Intervals

Negative

Performing Location
Roble Road Core Laboratory
Testing Schedule
Routine, 2 times per week