Test Directory
| Test Code | ||
| JHMSI | ||
| Test Name | ||
| Microsatellite Instability (MSI) PCR Analysis | ||
| CPT Codes | ||
| 81301 | ||
| Clinical Utility | ||
|
Microsatellite Instability (MSI) PCR Analysis is performed on template DNA extracted separately from areas of normal and tumor tissue microdissected from formalin-fixed paraffin-embedded (FFPE) tissue under microscopic control. PCR amplification is performed for the microsatellite loci BAT25, BAT26, D2S123, D5S346, and D17S250, as recommended by the revised guidelines issued by the International Collaborative Group on HNPCC and the National Cancer Institute (Dietmaier et al., Cancer Res 1997, 57:4749-56; Boland et al., Cancer Res 1998, 58:5248-57; Uman et al., JNCI 2004, 96:261-68). |
||
| Specimen and Container Info | ||
| Specimen Type: FFPE Block, unstained slides with matched H&E slide | ||
| Processing and Submission Instructions | ||
| Formalin-fixed and paraffin-embedded tissue: Slides: 7-10 sections of 10 μm unstained and 1 matched H&E slide. Please take adequate precautions to prevent cross-contamination with other specimens during slide cutting and transport. If it is not possible to send slides, the tumor block is acceptable, although not preferred. Multiple different tests can be performed on the same specimen. | ||
| Transport Instructions | ||
| Transport to the laboratory at room temperature. | ||
| Stability Requirements | ||
|
||
| Causes for Rejection | ||
| Specimens with less than 10% tumor content will be rejected.Do not send decalcified specimens, cytology smears or samples from hematologic malignancies. | ||
| Methodology | ||
| Next Gen Sequencing (NGS) | Multiplex Polymerase Chain Reaction (PCR) | ||
| Limitations | ||
|
Microsatellite Instability (MSI) PCR Analysis: PCR amplification is performed for the microsatellite loci BAT25, BAT26, D2S123, D5S346, and D17S250. |
||
| Performing Location | ||
| TJUH Center City | ||
| Alternate Names | ||
|
Microsatellite Instability (MSI) PCR Analysis (FFPE) |
||
| Reference Lab | ||
| TJUH Center City | ||
| Testing Schedule | ||
| Monday-Friday | ||
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
© 2026 HNL Lab Medicine. All rights reserved.