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Test Code
MITLY
Test Name
Mitogen-induced Lymphocyte Proliferation Panel (PHA, Con A, PWM)
CPT Codes
86353 X 3
Included Tests
  • Phytohemagglutinin (PHA)-induced Lymphocyte Proliferation
  • Concanavalin A (Con A)-induced Lymphocyte Proliferation
  • Pokeweed Mitogen (PWM)-induced Lymphocyte Proliferation
Expected Turnaround Time
Varies
Clinical Utility

Measurement of human lymphocytes' proliferative responses to various stimuli is a fundamental technique used to assess their biological status and functions. Mitogens, such as plant lectins phytohemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), are able to nonspecifically stimulate lymphocyte proliferation and used to evaluate patient immune responsiveness.
Specimen and Container Info

Specimen Type: Whole Blood

Collection Container: Green Top (Sodium Heparin)

Desired Specimen Volume: 10 mL

Collection Instructions

Testing requires special attention to specimen collection and shipping in order to ensure the integrity of the sample. Testing is by appointment only.

  1. Collect a full green top (sodium heparin) tube via venipuncture between 8 a.m - 12 p.m Monday - Thursday only.
Processing and Submission Instructions
  1. A scheduled courier is required for specimen transport.
  2. Transport 10 mL of whole blood in the original collection container.
  3. Label tube with a "room temperature" sticker.
Transport Instructions

Transport to the laboratory at room temperature.
Stability Requirements

Room Temperature

48 hours
Causes for Rejection
  • Hemolysis 
  • Clotted specimens
  • Samples collected in lithium heparin tubes
Methodology
Cell Culture (CC) & Scintillation Counter
Reference Intervals

See patient report.
Alternate Names
  • Antigen-induced Cell Proliferation
  • Mitogen-induced Cell Proliferation
  • Tuberculin PPD (TB PPD)
Reference Lab
Quest Diagnostics Nichols Institute
Testing Schedule
Routine, 4 times per week
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
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