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Test Code
MPL
Test Name
MPL Mutation Analysis
CPT Codes
81339
Included Tests

Next-generation sequencing of exon 10 of the MPL gene to detect all possible mutations, including those at the W515 and S505 codons.

Expected Turnaround Time
7 days
Clinical Utility

MPL W515 mutations are present in JAK2-negative patients with primary myelofibrosis (PMF) or essential thrombocythemia (ET) at a frequency of approximately 1-5%, respectively. The S505 mutation is usually detected in patients with familial essential thrombocythemia. Mutation analysis helps differentiate reactive conditions from myeloproliferative neoplasms (MPNs).
Specimen and Container Info

Specimen

Container

Preferred Volume

Minimum Volume

Peripheral Blood

Lavender Top (EDTA)

5 mL

3 mL

Bone Marrow

Lavender Top (EDTA)

2 mL

1 mL
Processing and Submission Instructions

Ship same day as drawn whenever possible. Specimens less than 72 hours old are preferred.
Transport Instructions

Transport to the laboratory refrigerated.
Stability Requirements

Refrigerated

72 hours
Causes for Rejection
  • Frozen specimens
  • Fixed specimens
  • Incorrect collection container
Methodology
Next Gen Sequencing (NGS)
Performing Location
Roble Road Core Laboratory
Alternate Names
  • Myeloproliferative Leukemia Protein
  • MPL 515/505 Mutation Analysis
  • MPL Mutation analysis by NGS
Reference Lab
Neogenomics Laboratories, Inc.
Testing Schedule
Routine, Monday - Friday no holidays
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
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