Test Directory

The Mumps Virus PCR test is used to confirm mumps infection in patients with compatible symptoms of mumps infection, including parotitis. Samples for Mumps Virus PCR should be collected as soon as practical after symptom onset.

Specimen Type: Buccal Swab

Collection Container: Viral Transport Media (VTM)

Alternate Collection Container: Universal Transport Media (UTM)

Desired Specimen Volume: 1 swab

1. Collect a buccal swab specimen by massaging the parotid gland for 30 seconds.
2. Using the swab included with the viral transport media collection kit, collect the sample by sweeping the swab between the cheek and gum from the upper molar to the lower molar for 10-15 seconds, while turning the swab. 
3. Sweep both sides with a single swab and place the swab into the VTM or UTM tube.

1. Ensure the VTM or UTM tube is capped tightly to avoid leakage.

Transport to the laboratory frozen. 

• Improperly submitted specimens
• Specimens not received in an approved VTM/UTM
• Swabs received in Liquid Amies (E-Swabs) or other bacterial swabs
• Specimens received without a swab
• Grossly leaking specimens
• Specimens received outside the stated stability.

See patient report.

A negative test does not rule out infection with mumps virus. Results should be interpreted in conjunction with clinical findings, and if required, serological testing. Specimens should be collected as soon as possible after the onset of symptoms to maximize the sensitivity of mumps virus detection. Improperly collected samples may result in false negative results. PCR assays may be impacted by viral variants and may result in reduced sensitivity or false negative results.

• Mumps, PCR, Swab