Test Directory

• MYC, 8q24.21
• IGH, 14q32.3

The t(8;14)(q32;q24) joins the cellular oncogenes C-MYC with sequences from immunoglobulin heavy chain gene enhancers leading to deregulated expression of C-MYC protein. This abnormality is characteristic of Burkitt lymphoma and may be seen with other chromosomal abnormalities in cases of large B-cell lymphoma. FISH can detect this translocation in either interphase or metaphase cells. FISH can detect this rearrangement in either interphase or metaphase cells.

Specimen: Formalin-Fixed Paraffin Embedded (FFPE) Tissue Block or 2 slides from FFPET block, cut at 4 micron thickness and mounted on charged lysine slides with accompanying H&E slide indicating the region(s) of interest (ROI), if the entire specimen is not tumor.

Specimens should have an ischemia time of < 1 hour and a formalin fixation time of

6 - 72 hours.

.

Transport to the laboratory at room temperature.

• Specimen arriving without any form of patient identification (unlabeled)
• FFPET sections that are not properly attached to the slide
• FFPET sections with excessive cracks, splits or deterioration that would inhibit analysis
• Broken FFPET slides

In a normal cell, the expected signal pattern is two red signals and two green signals. In an abnormal cell containing a typical translocation, the expected signal pattern is one red signal, one green signal, and two fused signals.

• FISH Translocation MYC/IGH
• IGH/MYC
• t(8;14)

• Association:
• Burkitt's Lymphoma