Test Directory

This assay measures the capability of neutrophils (PMN) to undergo oxidative metabolism to produce superoxide anion and hydrogen peroxide, as detected by oxidation of dihydrorhodamine (DHR). Patients with chronic granulomatous disease (CGD) are unable to oxidize DHR due to defects in oxidative capacity slowly decreases as the specimen age (hours post collection) increases. It is thus important that the reference range value appropriate for the specimen age be utilized when assessing the reported results.

Specimen Type: Whole Blood

Collection Container: Green Top (Sodium Heparin) for patient AND control

Desired Specimen Volume: 3 mL (patient) and 3 mL (control)

Testing requires special attention to specimen collection and shipping in order to ensure the integrity of the sample. Testing is by appointment only.

1. Collect a green top (sodium heparin) tube via venipuncture between 8 a.m - 12 p.m Monday - Thursday only.
2. Collect a green top (sodium heparin) tube via venipuncture for the control. Collect control specimen from a healthy individual unrelated to the patient at approximately the same time as and under similar conditions to the patient.

1. A scheduled courier is required for specimen transport.
2. Transport 3 mL of whole blood (patient) AND 3 mL of whole blood (control) in the original collection containers.
3. Label control specimen "Control for [Patient Name]".
4. Ensure collect date and time is on the control sample.
5. Label all tubes with a "room temperature" sticker.

Collect control specimen from a healthy individual unrelated to patient at approximately the same time as and under similar conditions to the patient.

Transport to the laboratory at room temperature.

• Samples received >72 hours after collection
• Hemolysis 
• Lipemia 
• Icterus 
• Clotted specimens

 See patient report.

• Neutrophil Function, Oxidative Burst