Test Directory

Test Code
NICOU
Test Name
Nicotine, Quantitative, Urine
CPT Codes
AMA 80323, CMS G0480
Expected Turnaround Time
3 - 5 days
Clinical Utility

This assay is used to determine the tobacco exposure status of the individual. Cotinine and 3 - OH -Cotinine are the primary metabolites of nicotine use and are detectable for several days after tobacco exposure. This assay will not distinguish between tobacco use and nicotine replacement products.

Specimen and Container Info

Preferred Specimen Type: Urine 

Preferred Collection Container: Plastic Urine Container (Preservative Free) 

Desired Specimen Volume: 10 mL 

Transport Instructions

Transport to the laboratory refrigerated.

Stability Requirements

Room Temperature

 7 Days 

Refrigerated

 7 Days 

Causes for Rejection

Urine specimens received in tubes containing preservatives will be rejected.

Methodology
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Reference Intervals

Analyte

Concentration (ng/mL)

Active tobacco use:

Cotinine

1000-8000

3-OH-Cotinine

3000-25,000

No tobacco use for greater than 2 weeks:

Cotinine

<50

3-OH-Cotinine

<120

Limitations

Analyte

Cutoff Concentration (ng/mL)

3-Hydroxycotinine

200

Cotinine

200

Performing Location
Roble Road Core Laboratory
Alternate Names
  • Cotinine
  • Nicotine Metabolite
  • Nicotine
Testing Schedule
Routine, 3 times per week