Test Directory

• BCL6 (3q27) Rearrangement
• MYC (8q24.21) Rearrangement
• IGH (14q32.3) Rearrangement
• IGH::BCL2, t(14;18)
• MALT1 (18q21.3) Rearrangement
• IGH::CCND1 (BCL1), t(11;14)
• ALK (2p23) Rearrangement
• MYC::IGH, t(8;11)
• Reflexed when appropriate: IGK and IGL

The NHL FISH panel is used for the detection of chromosome aberrations observed in lymphoma.

• Lithium heparin is not acceptable.
• Specimens must be collected in a sterile collection container.

Each specimen must be accompanied by a requisition form, script, electronic order or physician note. Each order must indicate the reason for testing (diagnosis).

• Transport to the laboratory at room temperature.
• Store specimen as whole blood, in original tube, at room temperature and deliver to lab within 24 hours.
• If specimen cannot be delivered within 24 hours, store in refrigerator. Do not freeze specimen.
• Specimens collected on the weekends must be refrigerated upon arrival at HNL Lab Medicine.

• Specimen frozen
• Specimen arriving without any form of patient identification (unlabeled)
• Specimen with attached needles
• Specimen arriving with broken or leaking collection tube
• Unfixed specimen treated with fixative

BCL6 ,3q27 Breakapart Probe-Rearrangement:

In a normal cell, the expected signal pattern is two fused signals. In an abnormal cell with a rearrangement, the expected signal pattern is one red signal, one green signal, and one fused signal.

MYC, 8q24.21 Breakapart Probe-Rearrangement

In a normal cell, the expected signal pattern is two fused signals. In an abnormal cell with a rearrangement, the expected signal pattern is one red signal, one green signal, and one fused signal.

IGH, 14q32.3 Breakapart Probe-Rearrangement

In a normal cell, the expected signal pattern is two fused signals. In an abnormal cell with a rearrangement, the expected signal pattern is one red signal, one green signal, and one fused signal.

IGH::BCL2, t(14;18) Translocation Probe

In a normal cell, the expected signal pattern is two red signals and two green signals. In an abnormal cell containing a typical translocation, the expected signal pattern is one red signal, one green signal, and two fused signals.

MALT1, 18q21.3 Breakapart Probe-Rearrangement

In a normal cell, the expected signal pattern is two fused signals. In an abnormal cell with a rearrangement, the expected signal pattern is one red signal, one green signal, and one fused signal.

BCL1 (CCND1)::IGH, t(11;14) Translocation Probe

In a normal cell, the expected signal pattern is two red signals and two green signals. In an abnormal cell containing a typical translocation, the expected signal pattern is one red signal, one green signal, and two fused signals.

ALK (2p23) Breakapart Probe-Rearrangement

In a normal cell, the expected signal pattern is two fused signals. In an abnormal cell with a rearrangement, the expected signal pattern is one red signal, one green signal, and one fused signal.

MYC::IGH t(8;14) Translocation Probe

In a normal cell, the expected signal pattern is two red signals and two green signals. In an abnormal cell containing a typical translocation, the expected signal pattern is one red signal, one green signal, and two fused signals.

• FISH NHL Panel,
• FISH Heme NHL

• Associations:
• Anaplastic Lymphoma Kinase (ALK)
• Burkitt's Lymphoma (BL)
• Diffuse Large B-cell Lymphoma (DLBCL)
• Follicular Lymphoma (FL)
• Mantle Cell Lymphoma (MCL)