Test Directory

• BCL6 , 3q27.3-q28 Rearrangement
• MYC ,8q24.21 Rearrangement
• IGH , 14q32.3 Rearrangement
• MALT1 ,18q21.31-q21.32 Rearrangement
• IGH::CCND1 (BCL1), t(11;14)
• ALK , 2p23 Rearrangement
• MYC::IGH, t(8;11)
• IGH::BCL2, t(14;18)
• Reflexed when appropriate: IGK and IGL

The NHL FISH panel is used for the detection of chromosome aberrations observed in lymphoma.

Specimen: Formalin-Fixed Paraffin Embedded (FFPE) Tissue Block or 9 slides from FFPET block, cut at 4 micron thickness and mounted on charged lysine slides with accompanying H&E slide indicating the region(s) of interest (ROI), if the entire specimen is not tumor.

Specimens should have an ischemia time of < 1 hour and a formalin fixation time of

6 - 72 hours.

Transport to the laboratory at room temperature.

• Specimen arriving without any form of patient identification (unlabeled)
• FFPET sections that are not properly attached to the slide
• FFPET sections with excessive cracks, splits or deterioration that would inhibit analysis
• Broken FFPET slides

BCL6, 3q27 Breakapart Probe- Rearrangement:

In a normal cell, the expected signal pattern is two fused signals. In an abnormal cell with a rearrangement, the expected signal pattern is one red signal, one green signal, and one fused signal.

MYC, 8q24.21 Breakapart Probe-Rearrangement

In a normal cell, the expected signal pattern is two fused signals. In an abnormal cell with a rearrangement, the expected signal pattern is one red signal, one green signal, and one fused signal.

IGH, 14q32.3 Breakapart Probe-Rearrangement

In a normal cell, the expected signal pattern is two fused signals. In an abnormal cell with a rearrangement, the expected signal pattern is one red signal, one green signal, and one fused signal.

IGH::BCL2, t(14;18) Translocation Probe

In a normal cell, the expected signal pattern is two red signals and two green signals. In an abnormal cell containing a typical translocation, the expected signal pattern is one red signal, one green signal, and two fused signals.

MALT1, 18q21.3 Breakapart Probe-Rearrangement

In a normal cell, the expected signal pattern is two fused signals. In an abnormal cell with a rearrangement, the expected signal pattern is one red signal, one green signal, and one fused signal.

BCL1(CCND1)::IGH, t(11;14) Translocation Probe

In a normal cell, the expected signal pattern is two red signals and two green signals. In an abnormal cell containing a typical translocation, the expected signal pattern is one red signal, one green signal, and two fused signals.

ALK, 2p23 Breakapart Probe-Rearrangement

In a normal cell, the expected signal pattern is two fused signals. In an abnormal cell with a rearrangement, the expected signal pattern is one red signal, one green signal, and one fused signal.

MYC::IGH, t(8;14) Translocation Probe

In a normal cell, the expected signal pattern is two red signals and two green signals. In an abnormal cell containing a typical translocation, the expected signal pattern is one red signal, one green signal, and two fused signals

• FISH NHL Panel
• FISH NHL, Tissue

• Associations:
• Anaplastic Lymphoma Kinase (ALK)
• Burkitt's Lymphoma (BL)
• Diffuse Large B-cell Lymphoma (DLBCL)
• Follicular Lymphoma (FL)
• Mantle Cell Lymphoma (MCL)