Test Directory

Test Code
NSFIB
Test Name
Nonalcoholic Steatohepatitis (NASH) FibroTest
CPT Codes
0003M
Included Tests
  • NASH-FibroTest, Interpretation
  • Apolipoprotein A1, S
  • Alpha-2-Macroglobulin, S
  • Haptoglobin, S
  • Alanine Aminotransferase (ALT), S
  • Gamma Glutamyltransferase (GGT), S
  • Bilirubin, Total, S
  • Aspartate Aminotransferase (AST), S
  • Cholesterol, Total, S
  • Triglycerides, S
  • Glucose, Fasting, P
Expected Turnaround Time
2 - 4 days
Clinical Utility

Diagnosis and the follow-up of liver fibrosis, steatosis, and inflammation.

Estimating hepatic fibrosisAssessing inflammation for metabolic diseases.

Assessing severity of nonalcoholic steatohepatitis (NASH) in patients with nonalcoholic fatty liver disease with steatosis (NAFLD).

Assessing steatosis or fatty liver.

Reassuring patients with steatosis only, without fibrosis.

Managing patients with severe injuries such as advanced fibrosis and NASH.

Specimen and Container Info

Specimen Type: Serum AND Plasma

Preferred Collection Container: Gold Top Serum Separator AND Gray Top (potassium oxalate/sodium fluoride)

Alternate Collection Container: Red Top, Plain AND Gray Top (potassium oxalate/sodium fluoride)

Desired Specimen Volume: 4 mL serum AND 1 mL plasma

Collection Instructions

Both serum and plasma are required for this test.

Serum:

  1. Collect a gold top, serum separator tube via venipuncture.

Plasma:

  1. Collect a gray top tube (potassium oxalate/sodium fluoride) via venipuncture.
Processing and Submission Instructions

Serum:

  1. Separate serum from cells within 2 hours of collection.
  2. Transfer 4 mL of serum to an amber aliquot tube.
  3. Centrifuged serum must be light protected within 4 hours of collection.
  4.  It is acceptable to draw the blood and then protect it from light after centrifugation as long as it's within 4 hours of collection.
  5. Label specimen as serum.

Plasma:

  1. Separate plasma from cells.
  2. Transfer 1 mL of plasma to a clear aliquot tube (screw cap).
  3.  Label specimen as plasma.
Patient Preparation
Fasting for at a minimum of 12 hours is required
Transport Instructions

Transport to the laboratory refrigerated. 

Stability Requirements

Room Temperature

Plasma: 7 days

Serum: 24 hours

Refrigerated

Plasma: 7 days

Serum: 7 days

Frozen

Plasma: 14 days

Serum: 14 days

Causes for Rejection
  • Patients <14 years of age
  • Gross hemolysis
  • Gross lipemia
  • Gross icterus
Methodology
Varies By Test
Reference Intervals

See patient report.

Limitations

This test is not offered for patients younger than 14 years of age. Testing will be canceled if a specimen is received from a patient in this age group.

Alternate Names
  • NASH
  • Nonalcoholic Steatohepatitis
  • Steatosis
  • Steato Test
Reference Lab
Mayo Clinic Laboratories
Testing Schedule
Routine, 5 times per week