Test Directory

• Summary
• Detection, 16S rDNA
• 16s Identification
• Detection, hsp65
• hsp65 Identification
• Detection, rpoB
• rpoB Identification
• Specimen DNA Extraction

Detection and identification of Nontuberculous Mycobacteria DNA. While Mycobacterium tuberculosis complex members are the most commonly known human pathogens, Non tuberculosis Mycobacteria (also known as environmental Mycobacteria, atypical Mycobacteria and Mycobacteria other than tuberculosis (MOTT) are also implicated in tuberculosis-like disease, localized lymphadenitis, gastrointestinal disease, and disseminated infections. Rapid and accurate identification of pathogenic Mycobacteria is important for correct therapeutic intervention against an infectious disease.

1. Collect tissue or fluid in a sterile container.

**MICRO AND INFECTIOUS DISEASE USE ONLY**

Requires University of Washington Molecular Microbiology Test Requisition Form

1. Transport specimen in the original collection container.
2. For FFPE specimens, a pathology report is recommended to be sent with the sample.

Transport to the laboratory frozen. 

• Sputum
• Blood, serum, plasma, stool/rectal swabs
• No citrated or heparinized solutions
• Tissues floating in formalin
• Swab/fluid collected in tube containing agar

See patient report.

This Nontuberculous Mycobacteria DNA Detection test can detect and identify Nontuberculous Mycobacteria with 3 gene targets: 16S, hsp65, and rpoB.

The presence of non-Mycobacterial DNA in these targets can interfere with the ability to rule out Nontuberculous Mycobacteria. In this scenario, the corresponding target will be reported as "Indeterminate. Unable to rule out due to interfering templates."

Fresh tissue is the optimal specimen of choice, as it reduces the chance of introducing exogenous DNA templates or microorganisms during embedding/fixation. Formalin fixation dramatically reduces the sensitivity of the assays due to reduced template yield and quality.

• Nontuberculous Mycobacteria DNA Detection
• AFB PCR
• Atypical Mycobacteria PCR
• Broad range AFB PCR
• Broad range Mycobacteria PCR
• hsp65
• M. avium complex
• MAC
• MAI PCR
• Molecular AFB
• Molecular Mycobacteria
• Molecular Mycobacterium
• MOTT
• Mycobacterial identification
• Mycobacterial sequencing
• Mycobacterium identification
• Mycobacterium leprae
• Mycobacterium sequencing
• Nontuberculous Mycobacterium PCR
• rpoB, universal AFB PCR
• Universal Mycobacterium PCR