Test Directory

Assessment of drug susceptibility by nucleic acid sequencing of a patient's hepatitis C virus (HCV) to the NS5A inhibitors. Assay should be used for patients with documented HCV genotype 1.

Preferred Specimen Type: Plasma

Alternate Specimen Type: Serum

Preferred Collection Container: Lavender Top (EDTA)

Alternate Collection Container: White Top (EDTA) or Gold Top, Serum Separator

Desired Specimen Volume: 2 mL

1. Collect a lavender top (EDTA) tube via venipuncture.

1.  Separate plasma from cells within 6 hours of collection.
2. Transfer 2 mL of plasma to a LabCorp PP transpak frozen purple tube.
3. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.

Please indicate the patient's HCV genotype (1a or 1b) on the test request form. Submit a separate frozen specimen for each test ordered.

Transport to the laboratory frozen. 

• PPT(TM) not centrifuged
• Insufficient volume
• Heparinized plasma
• Nonfrozen specimens
• Specimens exposed to repeated freeze/thaw cycles
• Specimen received in "pop-top" or "snap-cap" tube

 See patient report.

This procedure may not be successful when the HCV viral load is <500 IU/mL. This assay requires an HCV genotype of either 1a or 1b.

• HCV Drug Resistance
• NS5A Resistance Analysis