Test Directory

Test Code
NS5B
Test Name
NS5B Drug Resistance
CPT Codes
87900, 87902
Expected Turnaround Time
10 - 14 days
Clinical Utility
Assessment of drug susceptibility and nucleic acid sequencing of a patient's hepatitis C virus (HCV) to the NS5B inhibitors. Assay should be used for patients with documented HCV genotype 1.
Specimen and Container Info

Preferred Specimen Type: Plasma

Alternate Specimen Type: Serum

Preferred Collection Container: White Top (EDTA); OR Lavender Top (EDTA)

Alternate Collection Container: Gold Top, Serum Separator

Desired Specimen Volume: 2 mL

Collection Instructions

Collect gold top, serum separator; white top (EDTA); or lavender tube (EDTA) via venipuncture. Centrifuge specimen within six hours of collection, remove serum/plasma, and transfer specimen to polypropylene screw-capped tube, and freeze.

Processing and Submission Instructions
Please indicate the patient's HCV genotype (1a or 1b) on the test request form.
Submit a separate frozen specimen for each test ordered.
Transport Instructions

Transport to the laboratory frozen. 

Causes for Rejection
Insufficient volume, heparanized plasma, nonfrozen specimens, specimens exposed to repeated freeze/thaw cycles, specimen received in "pop-top" or "snap-cap" tube.
Methodology
Polymerase Chain Reaction (Pcr) Amplification And Dna Next Generation Sequencing
Reference Intervals

 See patient report.

Reference Lab
Labcorp Of America Holdings