Test Directory

Test Code
PDAML
Test Name
Pedi AML FISH Profile
CPT Codes
88271x2, 88274x2, 88275x2
Included Tests
  • BCR/ABL1, t(9;22)
  • KMT2a (MLL), 11q23
Expected Turnaround Time
3 - 5 business days
Specimen and Container Info

Specimen

Containers

Volume

Bone Marrow

Preferred Collection Container: Green Top (Sodium Heparin)

Alternate Collection Container: Lavender Top (EDTA)

Desired Specimen Volume: 3mL

Leukemic Blood

Preferred Collection Container: Green Top (Sodium Heparin)

Alternate Collection Container: Lavender Top (EDTA)

Desired Specimen Volume: 5mL

Bone Marrow/ Leukemic Blood Smear

4-5 slides labeled with the specimen type.

Formalin-fixed paraffin embedded (FFPE) tissue block

4-5 unstained slides cut to 4-5 microns thick

Fixed Cell Suspension

A client fixed cell suspension may be submitted for testing as long as it is received in 3:1 Methanol: Glacial Acetic Acid.

Collection Instructions

Do not collect in lithium heparin.

Transport Instructions
  • Refrigerate bone marrow and blood specimens.
  • Use cold packs for transportation. Make sure cold packs are not in direct contact with the specimen.
  • All specimens should be shipped the day of collection. Delay in shipping may compromise cell viability and results.
  • Transport slides and tissues at room temperature. Air dry slides and send in a protective case to prevent damage.
Stability Requirements

Frozen

Do not freeze.

Causes for Rejection
  • Broken or dirty slides.
  • Excessive cellular debris or stained slides.
  • Frozen specimens.
  • Decalcified specimens.
Methodology
FISH, Fluorescence In-Situ Hybridization
Performing Location
Roble Road Core Laboratory
Alternate Names

Pediatric Acute Myelocytic Leukemia FISH Profile

Reference Lab
Integrated Oncology, New York
Testing Schedule
Routine, Monday - Friday no holidays