Test Directory

This assay is used as an aid in the diagnosis of individuals suspected of having congestive heart failure. The test is further indicated for the risk stratification of patients with acute coronary syndrome and congestive heart failure. The test may also serve as an aid in the assessment of increased risk of cardiovascular events and mortality in patients at risk for heart failure who have stable coronary artery disease.

Specimen Type: Serum

Preferred Collection Container: Gold Top, Serum Separator

Alternate Collection Container: Red Top, Plain

Desired Specimen Volume: 1 mL

1. Collect a gold top, serum separator tube via venipuncture.

1. Separate serum from cells.
2. Transfer 1 mL of serum to a clear aliquot tube (screw cap).

This test may exhibit interference when sample is collected from a person who is consuming a supplement with a high dose of biotin (also termed as vitamin B7 or B8, vitamin H, or coenzyme R). It is recommended to ask all patients who may be indicated for this test about biotin supplementation. Patients should be cautioned to stop biotin consumption at least 72 hours prior to the collection of a sample.

Transport to the laboratory frozen. 

• Citrate plasma specimen
• Grossly hemolyzed samples
• Improper labeling

See patient report.

NT‑proBNP values should be assessed in conjunction with other cardiovascular risk factors and clinical findings.

Interpreting results in the setting of comorbidities such as cardiac, pulmonary, and renal disease, which are likely to increase natriuretic peptides above current thresholds for HF, should be done with caution.

The accuracy of natriuretic peptides for the detection of HF is reduced in the setting of atrial fibrillation and sepsis, and careful interpretation is warranted.

• proBNP
• Propeptide of Brain Natriuretic Peptide