Test Directory

Clear All
Test Code
PGENE
Test Name
Prothrombin (G20210A) Mutation
CPT Codes
81240
Expected Turnaround Time
2 - 4 days
Clinical Utility

A prothrombin mutation is the second most inherited clotting abnormality, present in approximately 2% of the general population. This mutation results in increase production of the protein which can activate thrombin and increase clot formation. Detection and genotyping of the prothrombin mutation aids in prevention and treatment plans for blood clots.
Specimen and Container Info

Specimen Type: Whole Blood

Collection Container:  Lavender Top (EDTA)

Desired Specimen Volume:  1 mL

Minimum Acceptable Volume:  500 µL
Processing and Submission Instructions

Specimen can only be shared with Factor V Leiden R506Q Mutation Test.
Transport Instructions

Transport to the laboratory refrigerated. 
Stability Requirements

Room Temperature

24 Hours

Refrigerated

15 days

Frozen

 3 Months
Causes for Rejection
  • Any specimen with leakage on the outside of the container will be rejected. Ensure the lid is tightly closed.
  • Any specimen shared with another laboratory test, except Factor V Leiden R506Q mutation, will be rejected.
  • Any specimen collected in an outdated collection tube will be rejected.
Methodology
Polymerase Chain Reaction (PCR)
Reference Intervals

Prothrombin Gene Mutation Not Detected
Performing Location
Roble Road Core Laboratory
Alternate Names
  • Prothrombin Gene G20210G>A
  • Factor II Mutation
  • Factor II Variant
Testing Schedule
Routine, Monday - Friday no holidays
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
© 2026 HNL Lab Medicine. All rights reserved.