Test Directory

EIA methods have the added advantage of being able to simultaneously detect RF of IgG and IgA subclasses in addition to RF IgM and are not susceptible to prozone. It has become apparent that the specificity and predictive value of the RF test is substantially increased by the detection of all three RF isotypes.

Specimen Type: Serum

Collection Container: Red Top, Plain

Alternate Collection Container: Gold Top, Serum Separator

Desired Specimen Volume: 1 mL

1. Collect a red top, plain tube via venipuncture.

1. Separate serum from cells within one hour of collection.
2. Transfer 1 mL of serum to a clear aliquot tube (screw cap).

Transport to the laboratory refrigerated.

• Grossly hemolyzed
• Bacterial contamination
• Lipemic, icteric, non-serum specimen types

See patient report.