Test Directory

Test Code
SKAB
Test Name
Skin Autoantibody Profile
CPT Codes
88346TC, 88350TC
Included Tests
  • Cell Surface Antibody IgG
  • Basement Membrane IgG
  • Primate Split Skin IgG
  • Cell Surface Ab IgG4
  • Basement Membrane IgG4
  • Primate Split Skin IgG4
Expected Turnaround Time
2 - 7 days
Clinical Utility

Confirming the presence of IgG antibodies to diagnose of pemphigoid, pemphigus, epidermolysis bullosa acquisita, or bullous lupus erythematosus.

Specimen and Container Info

Specimen Type: Serum

Collection Container: Gold Top, Serum Separator

Alternate Collection Container: Red Top, Plain

Desired Specimen Volume: 2 mL

Collection Instructions
  1. Collect a gold top, serum separator tube via venipuncture.
Processing and Submission Instructions
  1. Separate serum from cells.
  2. Transfer 2 mL of serum to a clear aliquot tube (screw cap).
Transport Instructions

Transport to the laboratory refrigerated. 

Stability Requirements

Room Temperature

14 days

Refrigerated

14 days

Frozen

30 days
Causes for Rejection
  • Gross lipemia
Methodology
Indirect Immunofluorescence
Reference Intervals

 See patient report.

Limitations

Results should be interpreted in conjunction with clinical information, histologic pattern, and results of direct immunofluorescence (IF) study. In particular, the finding of low titer (< or =1:80) anti-cell surface antibodies should not be used alone (ie, without histologic or direct IF support) to confirm a diagnosis of pemphigus.

Alternate Names
  • Cutaneous Immunofluorescence Antibodies, IgG and IgG4
  • Basement Membrane Antibodies
  • Bullous Pemphigoid
  • Cicatricial Pemphigoid
  • CII, Serum
  • Circulating IgG Basement Membrane Zone Antibody
  • Circulating Intercellular Ab Study
  • Epidermal Cell Surface Antibody
  • Epidermal Fluorescence Antibody
  • Epidermolysis Bullosa Acquisita
  • Epithelial Antibody
  • Herpes gestationis
  • IMF - Epidermal Fluorescent Ab
  • Indirect Immunofluorescence
  • Intercellular Substance Antibody
  • Pemphigoid
  • Pemphigus
  • Skin Basement Membrane Antibodies
  • Split-Skin Assay
  • Lupus Erythematosus (LE)
  • Bullous Lupus Erythematosus
Reference Lab
Mayo Clinic Laboratories
Testing Schedule
Monday-Friday
Reflex Tests
CIFST