Test Directory
| Test Code | ||
| NGS22 | ||
| Test Name | ||
| Solid Tumor Gene Fusion Analysis by Sequencing, Including NTRK | ||
| CPT Codes | ||
| 81449 | ||
| Expected Turnaround Time | ||
| 13 - 14 days | ||
| Clinical Utility | ||
| Solid Tumor Gene Fusion Analysis by Sequencing, Including NTRK detects gene fusions involving 18 genes. Another 18 genes are targeted by the panel for expression and/or mutation analysis. | ||
| Specimen and Container Info | ||
| Specimen Type: FFPE Block, unstained slides with matched H&E Slide | ||
| Processing and Submission Instructions | ||
| Formalin-fixed and paraffin-embedded tissue: Slides cut in the following order: 12 sections of 4μm unstained, 1 H&E, 10 sections of 10μm unstained, 1 H&E. Please take adequate precautions to prevent cross-contamination with other specimens during slide cutting and transport. | ||
| Transport Instructions | ||
| Transport to the laboratory at room temperature. | ||
| Stability Requirements | ||
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| Causes for Rejection | ||
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Specimens with less than 10% tumor content will be rejected. Do not submit decalcified specimens, cytology smears or samples from hematologic malignancies. |
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| Methodology | ||
| Next Gen Sequencing (NGS) | ||
| Limitations | ||
|
DNA Analysis Genes: BRAF Extended Mutation Analysis (All Hotspots, Exons 11,15) EGFR Mutation Analysis (Exons 18-21 Including T790M) ERBB2 Mutation Analysis (Exons 8, 17,19,20,21) KRAS Extended Mutation Analysis (All Hotspots, Exons 2-4)18 RNA Genes - Exons covered by RNA fusion panel [reference transcript in brackets] ALK - exons 2, 4, 6, 10, 16-23, 25 [NM_004304] AXL - exons 5, 10, 14, 16-19 [NM_001699] BRAF - exons 7-12, 15 [NM_004333] CCND1 - exons 1-4 [NM_053056] EGFR - exons 8, 18-21 [NM_005228] FGFR1 - exons 2, 4, 6, 8-12, 17 [NM_015850] FGFR2 - exons 2, 5, 7-10, 17 [NM_000141] FGFR3 - exons 3, 5, 8-10, 17 [NM_000142] MET - exons 2, 4-6, 13-17, 21 [NM_000245] NRG1 - exons 1-3, 6 [NM_013956] NTRK1 - exons 2, 4, 6, 8-13 [NM_002529] NTRK2 - exons 5, 7, 9, 11-17 [NM_006180] NTRK3 - exons 4, 7, 10, 13-16 [NM_002530] PPARG - exons 1-3, 5 [NM_015869] RAF1 - exons 4-12 [NM_002880] RET - exons 2, 4, 6, 8-16 [NM_020975] ROS1 - exons 2, 4, 7, 31-38 [NM_002944] THADA - exons 24-30, 36, 37 [NM_022065] |
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| Performing Location | ||
| TJUH Center City | ||
| Alternate Names | ||
|
NGS Solid Tumor Gene Fusion Analysis by Sequencing, Including NTRK |
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| Reference Lab | ||
| TJUH Center City | ||
| Testing Schedule | ||
| Monday-Friday | ||
The American Medical Association (AMA) Current Procedural Terminology (CPT) codes published by HNL Lab Medicine are guidelines and are intended for informational purposes only. CPT coding is the exclusive responsibility of the billing entity. View the Terms.
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