Test Directory

Test Code
ASOT
Test Name
Streptolysin O Autoantibody
CPT Codes
86060
Expected Turnaround Time
3 - 7 days
Clinical Utility

Demonstration of acute or recent streptococcal infection. 85% of patients with acute rheumatic fever show increased Streptolysin O (ASOT) levels.

Specimen and Container Info

Preferred Specimen Type: Serum

Desired Specimen Volume: 2 mL

Preferred Collection Container: Gold Top, Serum Separator

Processing and Submission Instructions

Separator tubes: Contains clot activator and gel for separating serum from cells, but not anticoagulant. Separate serum from cells as soon as the clot has formed (30 minutes). If specimen is centrifuged before clotting is complete, a fibrin clot will form on top of the cell. This may cause the gel barrier to not be intact and could cause improper separation of serum and cells, possibly affecting test results.

Transport Instructions

Transport refrigerated.

Stability Requirements

Refrigerated

7 days

Frozen

<20°C for long-term storage.

Freeze/Thaw Cycles

Avoid multiple freeze/thaw cycles.

Methodology
Turbidimetric
Reference Intervals

≤ 200 IU/mL

Performing Location
Roble Road Core Laboratory
Testing Schedule
Routine, 3 times per week