Test Directory

Test Code
QTEST
Test Name
Testosterone, Free and Total
CPT Codes
84402, 84403
Included Tests
  • Testosterone, Total, MS
  • Testosterone, Free
Expected Turnaround Time
3 - 4 days
Clinical Utility

Helpful in assessing testicular function in prepubescent hypogonadal males and in managing hirsutism, virilization in females.

Specimen and Container Info

Preferred Specimen Type: Serum

Alternate Specimen Type: Plasma

Preferred Collection Container: Red Top, Plain

Alternate Collection Container: Gold Top, Serum Separator or Green Top (Sodium or Lithium Heparin)

Desired Specimen Volume: 1.8 mL

Collection Instructions
  1. Collect a red top, plain tube via venipuncture.
  2. Allow specimen to clot at room temperature.
Processing and Submission Instructions
  1. Separate serum from cells.
  2. Transfer 1.8 mL of serum to a clear aliquot tube (screw cap).
Transport Instructions

Transport to the laboratory refrigerated. 

Stability Requirements

Room Temperature

 7 days

Refrigerated

21 days

Frozen

2 years

Causes for Rejection
  • Gross hemolysis 
  • Grossly lipemic
Methodology
Chromatography/Mass Spectrometry | Equilibrium Dialysis
Reference Intervals

 See patient report.

Alternate Names
  • Testosterone, Free (Dialysis) and Total, MS
  • Free & Total Testosterone
  • Free and Unbound Testosterone
Reference Lab
Quest Diagnostics Nichols Institute
Testing Schedule
Routine, daily